The protection of selenium against cadmium-induced cytotoxicity via the heat shock protein pathway in chicken splenic lymphocytes.

ABSTRACT: Cadmium (Cd) is a heavy metal that poses a hazard to animal health due to its toxicity. Selenium (Se) is an important nutritional trace element. However, the potential protective effects of Se against Cd-induced toxicity remain to be elucidated. To investigate the cytotoxicity of Cd on bird immunocytes in vitro and the protective effects of Se against exposure to Cd, chicken splenic lymphocytes received Cd (10⁻⁶ mol/L), Se (10⁻⁷ mol/L), and the mixture of 10⁻⁷ mol/L Se and 10⁻⁶ mol/L Cd and were incubated for 12 h, 24 h, 36 h, 48 h, respectively. The transcription of heat shock protein (HSP) 27, HSP40, HSP60, HSP70 and HSP90 mRNA was tested by fluorescence quantitative PCR. The results showed that the mRNA expression of HSPs exposed to 10⁻⁶ mol/L Cd showed a sustained decrease at 12-48 h e

SUBMITTER: Chen X 

PROVIDER: S-EPMC6268861 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature