Evaluating cellularity and structural connectivity on whole brain slides using a custom-made digital pathology pipeline.

ABSTRACT:

Aims

In brain research, the histopathological examination of coronar whole-brain slides provides important insights into spatial disease characteristics. Regarding brain tumor research, this enables visualization of tumor heterogeneity, infiltration patterns and the relationship with the surrounding brain parenchyma. The precise correlation between radiological imaging and post-mortem brains is of special interest.

New method

We developed a wide-field slide scanner, comprising a microscope, a high-precision remotely controllable x-y-stage, a camera and a computer workstation, for automatically scanning uncommonly large formats. We analyzed whole brain slides of three patients and constructed cellularity heatmaps and fiber tract maps using a custom-made image processing pipel

SUBMITTER: Roetzer T 

PROVIDER: S-EPMC6269083 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature