Assessment of renal fibrosis in murine diabetic nephropathy using quantitative magnetization transfer MRI.

ABSTRACT:

Purpose

Renal fibrosis is a hallmark of progressive renal disease; however, current clinical tests are insufficient for assessing renal fibrosis. Here we evaluated the utility of quantitative magnetization transfer MRI in detecting renal fibrosis in a murine model of progressive diabetic nephropathy (DN).

Methods

The db/db eNOS-/- mice, a well-recognized model of progressive DN, and normal wild-type mice were imaged at 7T. The quantitative magnetization transfer data were collected in coronal plane using a 2D magnetization transfer prepared spoiled gradient echo sequence with a Gaussian-shaped presaturation pulse. Parameters were derived using a two-pool fitting model. A normal range of cortical pool size ratio (PSR) was defined as Mean±2SD of wild-type kidneys (N = 20). The

SUBMITTER: Wang F 

PROVIDER: S-EPMC6269231 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature