Mycoplasma contamination of leukemic cell lines alters protein expression determined by reverse phase protein arrays.

ABSTRACT: Mycoplasma contamination is a major problem in cell culturing, potentially altering the results of cell line-based experiments in largely uncharacterized ways. To define the consequences of mycoplasma infection at the level of protein expression we utilized the reverse phase protein array technology to analyze the expression of 235 proteins in mycoplasma infected, uninfected post treatment, and never-infected leukemic cell lines. Overall, protein profiles of cultured cells remained relatively stable after mycoplasma infection. However, paired comparisons for individual proteins identified that 18.7% of the proteins significantly changed between the infected and the never-infected cell line samples, and that 14.0% of the proteins significantly altered between the infected and the post treat

SUBMITTER: Hoff FW 

PROVIDER: S-EPMC6269355 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature