Facile development of medium optimization for antibody production: implementation in spinner flask and hollow fiber reactor.

ABSTRACT: Most bio-industrial mammalian cells are cultured in serum-free media to achieve advantages, such as batch consistency, suspended growth, and simplified purification. The successful development of a serum-free medium could contribute to a reduction in the experimental variation, enhance cell productivity, and facilitate biopharmaceuticals production using the cell culture process. Commercial serum-free media are also becoming more and more popular. However, the cell line secrets its own recombinant product and has special nutritional requirements. How can the composition of the proprietary medium be adjusted to support the specific cell's metabolism and recombinant protein? This article uses statistical strategies to modify the commercial medium. A design of experiments is adopted to optimi

SUBMITTER: Liu CH 

PROVIDER: S-EPMC6269363 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature