Efflux pump-mediated resistance to antifungal compounds can be prevented by conjugation with triphenylphosphonium cation.

ABSTRACT: Antifungal resistance due to upregulation of efflux pumps is prevalent in clinical Candida isolates. Potential efflux pump substrates (PEPSs), which are active against strains deficient in efflux pumps but inactive against wild-type strains, are usually missed in routine antifungal screening. Here we present a method for identification of PEPSs, and show that conjugation with mitochondria-targeting triphenylphosphonium cation (TPP⁺) can enhance or restore the compounds' antifungal activity. The screening method involves co-culturing a wild-type C. albicans strain and a Cdr efflux pump-deficient strain, labelled with different fluorescent proteins. We identify several PEPSs from a library of natural terpenes, and restore their antifungal activity against wild-type and azole-resis

SUBMITTER: Chang W 

PROVIDER: S-EPMC6269435 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature