3D microdevices that perform sample purification and multiplex qRT-PCR for early cancer detection with confirmation of specific RNAs.

ABSTRACT: MicroRNA expression analysis is an important screening tool for the early detection of cancer. In this study, we developed two portable three-dimensional microdevices for multiple singleplex RNA expression analysis by microRNA purification and qRT-PCR as a prototype for point-of-care testing. These microdevices are composed of several types of modules termed 'chemical IC chips'. We successfully reduced the heating area and fluorescence observation area, reduced the energy required for the reaction, and improved the portability of all systems in the devices. The purification microdevice could purify the microRNA from the sample using the FTA elute card system. The disposable reactor module mounted on both devices was easily fabricated by deforming a 100-μm-thick polypropylene film using an

SUBMITTER: Kimura Y 

PROVIDER: S-EPMC6269476 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature