Diffracted X-ray Blinking Tracks Single Protein Motions.

ABSTRACT: Single molecule dynamics studies have begun to use quantum probes. Single particle analysis using cryo-transmission electron microscopy has dramatically improved the resolution when studying protein structures and is shifting towards molecular motion observations. X-ray free-electron lasers are also being explored as routes for determining single molecule structures of biological entities. Here, we propose a new X-ray single molecule technology that allows observation of molecular internal motion over long time scales, ranging from milliseconds up to 10³ seconds. Our method uses both low-dose monochromatic X-rays and nanocrystal labelling technology. During monochromatic X-ray diffraction experiments, the intensity of X-ray diffraction from moving single nanocrystals appears to

SUBMITTER: Sekiguchi H 

PROVIDER: S-EPMC6269541 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature