The effect of chitin size, shape, source and purification method on immune recognition.

ABSTRACT: The animal immune response to chitin is not well understood and needs to be investigated further. However, this is a challenging topic to study because of the technical difficulties in purifying chitin, and because this material usually comes associated with contaminating components that can activate the immune system. In this study, improvements to previously described purification protocols were investigated for chitin obtained from different sources, including commercial shellfish, Candida albicans yeast and hyphal cell walls, as well as cell walls of the filamentous fungi Aspergillus fumigatus and Mucor circinelloides. The immune response to these different chitin preparations was tested using human peripheral blood mononuclear cells. In agreement with previous literature, small chitin

SUBMITTER: Alvarez FJ 

PROVIDER: S-EPMC6271096 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature