A starting point for fluorescence-based single-molecule measurements in biomolecular research.

ABSTRACT: Single-molecule fluorescence techniques are ideally suited to provide information about the structure-function-dynamics relationship of a biomolecule as static and dynamic heterogeneity can be easily detected. However, what type of single-molecule fluorescence technique is suited for which kind of biological question and what are the obstacles on the way to a successful single-molecule microscopy experiment? In this review, we provide practical insights into fluorescence-based single-molecule experiments aiming for scientists who wish to take their experiments to the single-molecule level. We especially focus on fluorescence resonance energy transfer (FRET) experiments as these are a widely employed tool for the investigation of biomolecular mechanisms. We will guide the reader through the

SUBMITTER: Gust A 

PROVIDER: S-EPMC6271140 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature