Rapid identification of antioxidant compounds of Genista saharae Coss. & Dur. by combination of DPPH scavenging assay and HPTLC-MS.

ABSTRACT: Genista species are sources of antioxidant phenolic compounds such as O- and C-glycosylflavonoids and isoflavonoids. A combination of a DPPH scavenging assay with HPTLC-MS, a fast and efficient method for identification of bioactive compounds, has been applied for evaluation of the radical scavenging activity of metabolites from Genista saharae Coss. & Dur. Different organs collected at various periods have been compared. Identification of antioxidant compounds was obtained by elution of the major DPPH-inhibition zones. The resulting HPTLC-MS analysis under moderately polar conditions, coupled to the DPPH results led to the putative identification of two antioxidant isoflavone aglycones: 3',4',5,7-tetrahydroxyisoflavone (1) and ficuisoflavone (3), whereas polar migration conditions led

SUBMITTER: Meriane D 

PROVIDER: S-EPMC6271480 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature