Active K-RAS induces the coherent rotation of epithelial cells: A model for collective cell invasion in vitro.

ABSTRACT: At the invasive front of adenocarcinomas, single cells and multicellular structures exist; the latter include glands and cell clusters, such as tumor buddings and poorly differentiated clusters. Recent reports suggest the importance of collective cell migration in metastasis; however, it is technically difficult to observe the movement of multicellular structures in vivo. We utilized MDCK cells as a model for epithelial cells and established a method to quantify their motility in 3D structures in vitro. A single MDCK cell grows as a cell cluster in the gel and later proliferates and forms a cyst. Active K-RAS expression induced rotation of both the cell clusters and the cysts. The rotation speed of cell clusters was 4 times higher than that of cysts. The screening of inhibitors for their e

SUBMITTER: Hirata E 

PROVIDER: S-EPMC6272113 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature