Reinforce the antitumor activity of CD8⁺ T cells via glutamine restriction.

ABSTRACT: The antitumor activity of activated CD8+ T cells in the tumor microenvironment seems to be limited due to their being metabolically unfit. This metabolic unfitness is closely associated with T-cell exhaustion and impairment of memory formation, which are barriers to successful antitumor adoptive immunotherapy. We therefore assessed the role of glutamine metabolism in the antitumor activity of CD8⁺ T cells using a tumor-inoculated mouse model. The adoptive transfer of tumor-specific CD8⁺ T cells cultured under glutamine-restricted (dGln) conditions or CD8⁺ T cells treated with specific inhibitors of glutamine metabolism efficiently eliminated tumors and led to better survival of tumor-inoculated mice than with cells cultured under control (Ctrl) conditions.

SUBMITTER: Nabe S 

PROVIDER: S-EPMC6272119 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature