Inhibition of Urease by Disulfiram, an FDA-Approved Thiol Reagent Used in Humans.

ABSTRACT: Urease is a nickel-dependent amidohydrolase that catalyses the decomposition of urea into carbamate and ammonia, a reaction that constitutes an important source of nitrogen for bacteria, fungi and plants. It is recognized as a potential antimicrobial target with an impact on medicine, agriculture, and the environment. The list of possible urease inhibitors is continuously increasing, with a special interest in those that interact with and block the flexible active site flap. We show that disulfiram inhibits urease in Citrullus vulgaris (CVU), following a non-competitive mechanism, and may be one of this kind of inhibitors. Disulfiram is a well-known thiol reagent that has been approved by the FDA for treatment of chronic alcoholism. We also found that other thiol reactive compounds (l-capt

SUBMITTER: Diaz-Sanchez AG 

PROVIDER: S-EPMC6274061 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature