LC-MS/MS Validation Analysis of Trastuzumab Using dSIL Approach for Evaluating Pharmacokinetics.

ABSTRACT: Quantitative targeted proteomics based approaches deploy state-of-the-art Liquid chromatography tandem mass spectrometry LC-MS technologies and are evolving as a complementary technique to standard ligand-binding based assays. Advancements in MS technology, which have augmented the specificity, selectivity and sensitivity limits of detection and freedom from antibody generation, have made it amicable towards various clinical applications. In our current work, a surrogate peptide based quantitative proteomics assessment is performed by selecting specific signature peptides from the complementary determining region CDR region of trastuzumab (Herclon^®, Roche products in India). We developed a double Stable Isotope Label (dSIL) approach by using two different surrogate peptides to e

SUBMITTER: Budhraja RH 

PROVIDER: S-EPMC6274275 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature