A Metagenomic Advance for the Cloning and Characterization of a Cellulase from Red Rice Crop Residues.

ABSTRACT: Many naturally-occurring cellulolytic microorganisms are not readily cultivable, demanding a culture-independent approach in order to study their cellulolytic genes. Metagenomics involves the isolation of DNA from environmental sources and can be used to identify enzymes with biotechnological potential from uncultured microbes. In this study, a gene encoding an endoglucanase was cloned from red rice crop residues using a metagenomic strategy. The amino acid identity between this gene and its closest published counterparts is lower than 70%. The endoglucanase was named EglaRR01 and was biochemically characterized. This recombinant protein showed activity on carboxymethylcellulose, indicating that EglaRR01 is an endoactive lytic enzyme. The enzymatic activity was optimal at a pH of 6.8 and a

SUBMITTER: Meneses C 

PROVIDER: S-EPMC6274478 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature