Autoantibodies to IgE and FcεRI and the natural variability of spleen tyrosine kinase expression in basophils.

ABSTRACT: BACKGROUND:Secretion from human basophils and mast cells requires spleen tyrosine kinase (SYK) activity, but SYK expression is highly variable in the general population, and this variability predicts the magnitude of IgE-mediated secretion. One known mechanism of modulating SYK expression in human basophils is aggregation of FcεRI. OBJECTIVE:This study examines the possibility that functional autoantibodies are present in a wide variety of subjects and, in particular, subjects whose basophils poorly express SYK. It also tests whether any found antibodies could modulate SYK expression in maturing basophils and whether interaction with FcγRIIb/CD32b modulates the effect. METHODS:An experimental algorithm for classifying the nature of histamine release induced by serum from 3 classes of subje

SUBMITTER: MacGlashan D 

PROVIDER: S-EPMC6274596 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature