Benefits of NaCl addition for time-of-flight secondary ion mass spectrometry analysis including the discrimination of diacylglyceride and triacylglyceride ions.

ABSTRACT:

Rationale

Diacylglycerides (DAGs) and triacylglycerides (TAGs) are two important lipid classes present in all mammalian cells that share similar chemical structures but differ in biological function in cells and tissues. Differentiation of these two species during time-of-flight secondary ion mass spectrometry (ToF-SIMS) analysis is therefore important, but has been difficult due to the formation of DAG-like ions during the ionization process of TAGs.

Methods

We investigated the use of salt adduct formation as a quick and simple method to determine the origin of the DAG-like ions in ToF-SIMS spectra. NaCl was added to lipid standards of a DAG and a TAG and differences in fragmentation patterns were identified. The salt was then applied to prepared tissue samples by spraying

SUBMITTER: Samfors S 

PROVIDER: S-EPMC6274607 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature