The Melting Diagram of Protein Solutions and Its Thermodynamic Interpretation.

ABSTRACT: Here we present a novel method for the characterization of the hydration of protein solutions based on measuring and evaluating two-component wide-line ¹H NMR signals. We also provide a description of key elements of the procedure conceived for the thermodynamic interpretation of such results. These interdependent experimental and theoretical treatments provide direct experimental insight into the potential energy surface of proteins. The utility of our approach is demonstrated through the examples of two proteins of distinct structural classes: the globular, structured ubiquitin; and the intrinsically disordered ERD10 (early response to dehydration 10). We provide a detailed analysis and interpretation of data recorded earlier by cooling and slowly warming the protein solutions through th

SUBMITTER: Tompa K 

PROVIDER: S-EPMC6274677 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature