Intravenous Delivery of piggyBac Transposons as a Useful Tool for Liver-Specific Gene-Switching.

ABSTRACT: Hydrodynamics-based gene delivery (HGD) is an efficient method for transfecting plasmid DNA into hepatocytes in vivo. However, the resulting gene expression is transient, and occurs in a non-tissue specific manner. The piggyBac (PB) transposon system allows chromosomal integration of a transgene in vitro. This study aimed to achieve long-term in vivo expression of a transgene by performing hepatocyte-specific chromosomal integration of the transgene using PB and HGD. Using this approach, we generated a novel mouse model for a hepatic disorder. A distinct signal from the reporter plasmid DNA was discernible in the murine liver approximately two months after the administration of PB transposons carrying a reporter gene. Then, to induce the hepatic disorder, we first administered mice

SUBMITTER: Nakamura S 

PROVIDER: S-EPMC6274756 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature