Automated Intracellular Calcium Profiles Extraction from Endothelial Cells Using Digital Fluorescence Images.

ABSTRACT: Endothelial cells perform a wide variety of fundamental functions for the cardiovascular system, their proliferation and migration being strongly regulated by their intracellular calcium concentration. Hence it is extremely important to carefully measure endothelial calcium signals under different stimuli. A proposal to automate the intracellular calcium profiles extraction from fluorescence image sequences is presented. Digital image processing techniques were combined with a multi-target tracking approach supported by Kalman estimation. The system was tested with image sequences from two different stimuli. The first one was a chemical stimulus, that is, ATP, which caused small movements in the cells trajectories, thereby suggesting that the bath application of the agonist does not genera

SUBMITTER: Sanchez-Tecuatl M 

PROVIDER: S-EPMC6274978 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature