Fabrication of an Open Microfluidic Device for Immunoblotting.

ABSTRACT: Given the wide adoption of polydimethylsiloxane (PDMS) for the rapid fabrication of microfluidic networks and the utility of polyacrylamide gel electrophoresis (PAGE), we develop a technique for fabrication of PAGE molecular sieving gels in PDMS microchannel networks. In developing the fabrication protocol, we trade-off constraints on materials properties of these two polymer materials: PDMS is permeable to O₂ and the presence of O₂ inhibits the polymerization of polyacrylamide. We present a fabrication method compatible with performing PAGE protein separations in a composite PDMS-glass microdevice, that toggles from an "enclosed" microchannel for PAGE and blotting to an "open" PA gel lane for immunoprobing and readout. To overcome the inhibitory effects of O₂

SUBMITTER: Abdel-Sayed P 

PROVIDER: S-EPMC6275090 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature