The effect of insulin on equine lamellar basal epithelial cells mediated by the insulin-like growth factor-1 receptor.

ABSTRACT:

Background

In horses and ponies, insulin dysregulation leading to hyperinsulinemia may be associated with increased risk of laminitis, and prolonged infusion of insulin can induce the condition. It is unclear whether insulin may have a direct or indirect effect on the lamellar tissues. Insulin is structurally related to insulin-like growth factor (IGF-1), and can bind the IGF-1 receptor, albeit at a lower affinity than IGF-1.

Methods

Immunohistochemistry was performed on formalin-fixed lamellar tissue sections from six normal horses, euthanised for non-research purposes, using an anti-IGF-1 receptor antibody. In further studies, lamellar epithelial cells were obtained by collagenase digestion from the hooves of 18 normal horses, also euthanised for non-research purposes, and

SUBMITTER: Baskerville CL 

PROVIDER: S-EPMC6275117 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature