AcI Actinobacteria Assemble a Functional Actinorhodopsin with Natively Synthesized Retinal.

ABSTRACT: Freshwater lakes harbor complex microbial communities, but these ecosystems are often dominated by acI Actinobacteria Members of this cosmopolitan lineage are proposed to bolster heterotrophic growth using phototrophy because their genomes encode actino-opsins (actR). This model has been difficult to validate experimentally because acI Actinobacteria are not consistently culturable. Based primarily on genomes from single cells and metagenomes, we provide a detailed biosynthetic route for members of acI clades A and B to synthesize retinal and its carotenoid precursors. Consequently, acI cells should be able to natively assemble light-driven actinorhodopsins (holo-ActR) to pump protons, unlike many bacteria that encode opsins but may need to exogenously obtain retinal b

SUBMITTER: Dwulit-Smith JR 

PROVIDER: S-EPMC6275354 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature