Transcriptomic depression of immunological synapse as a signature of ventilator-associated pneumonia.

ABSTRACT:

Background

Ventilator-associated pneumonia (VAP) is one of the most commonly encountered intensive care unit (ICU) acquired infections worldwide. The objective of the study was to identify the immune alteration occurring in patients suffering from VAP at the transcriptomic level and explore its potential use for clinical diagnoses of this disease.

Methods

We performed a prospective observational study in five medical ICUs. Immunological gene expression profiles in the blood of VAP patients were compared with those of controls by using whole transcriptome microarrays and droplet digital polymerase chain reaction (ddPCR) in the first 24 hours following diagnosis.

Results

VAP patients showed significantly lower expression levels of HLA-DOA, HLA-DMA, HLA-DMB, ICOS, ICOSLG

SUBMITTER: Almansa R 

PROVIDER: S-EPMC6275407 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature