Comparison of miRNA Profiles of Cord Blood Stem Cells in Identical and Fraternal Twins.

ABSTRACT:

Objective

The role of epigenetic in regulating of the gene expression profile the embryo has been documented. MicroRNAs (miRNAs) are one of these epigenetic mechanisms. Twins are valuable models in determining the relative contributions of genetics and the environment. In this study, we compared differences in the expression levels of 44 miRNAs in hematopoietic stem cells (HSCs) of identical twins to that of fraternal twins as a controls.

Materials and methods

In this experimental study, CD133⁺ HSCs were isolated from cord blood of identical and fraternal twins via magnetic-activated cell sorting (MACS). Variation in of gene expression levels of 44 miRNAs were evaluated using quantitative reverse transcription-polymerase chain reaction (qRT-PCR).

Results

Si

SUBMITTER: Ajami M 

PROVIDER: S-EPMC6275421 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature