Detection of Mycoplasma Contamination of Cell Culture by A Loop-Mediated Isothermal Amplification Method.

ABSTRACT:

Objective

Mycoplasmas are major contaminants of cell culture and affect in vitro biological and diagnostic tests. Mycoplasma detection is conducted using culture and molecular methods. These methods vary in terms of accuracy, reliably and sensitivity. Loop-mediated isothermal amplification (LAMP) is used to amplify target DNA in a highly specific and rapid manner. This study aimed to develop a LAMP method for rapid detection of Mycoplasma in culture samples.

Materials and methods

In this descriptive laboratory study, for LAMP detection of Mycoplasma contaminations in cell culture, we used primers specifically designed for targeting the 16S rRNA conserved gene of Mycoplasma spp. For a positive control structure, 16S rRNA amplified based on PCR, was cloned in a plasmid vector

SUBMITTER: Soheily Z 

PROVIDER: S-EPMC6275423 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature