Structure-based design of a quadrivalent fusion glycoprotein vaccine for human parainfluenza virus types 1-4.

ABSTRACT: Parainfluenza virus types 1-4 (PIV1-4) are highly infectious human pathogens, of which PIV3 is most commonly responsible for severe respiratory illness in newborns, elderly, and immunocompromised individuals. To obtain a vaccine effective against all four PIV types, we engineered mutations in each of the four PIV fusion (F) glycoproteins to stabilize their metastable prefusion states, as such stabilization had previously enabled the elicitation of high-titer neutralizing antibodies against the related respiratory syncytial virus. A cryoelectron microscopy structure of an engineered PIV3 F prefusion-stabilized trimer, bound to the prefusion-specific antibody PIA174, revealed atomic-level details for how introduced mutations improved stability as well as how a single PIA174 antibody recogniz

SUBMITTER: Stewart-Jones GBE 

PROVIDER: S-EPMC6275507 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature