Genome-wide RNA structurome reprogramming by acute heat shock globally regulates mRNA abundance.

ABSTRACT: The heat shock response is crucial for organism survival in natural environments. RNA structure is known to influence numerous processes related to gene expression, but there have been few studies on the global RNA structurome as it prevails in vivo. Moreover, how heat shock rapidly affects RNA structure genome-wide in living systems remains unknown. We report here in vivo heat-regulated RNA structuromes. We applied Structure-seq chemical [dimethyl sulfate (DMS)] structure probing to rice (Oryza sativa L.) seedlings with and without 10 min of 42 °C heat shock and obtained structural data on >14,000 mRNAs. We show that RNA secondary structure broadly regulates gene expression in response to heat shock in this essential crop species. Our results indicate significant heat-induced eleva

SUBMITTER: Su Z 

PROVIDER: S-EPMC6275526 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature