Monomerization of far-red fluorescent proteins.

ABSTRACT: Anthozoa-class red fluorescent proteins (RFPs) are frequently used as biological markers, with far-red (λ_em ∼ 600-700 nm) emitting variants sought for whole-animal imaging because biological tissues are more permeable to light in this range. A barrier to the use of naturally occurring RFP variants as molecular markers is that all are tetrameric, which is not ideal for cell biological applications. Efforts to engineer monomeric RFPs have typically produced dimmer and blue-shifted variants because the chromophore is sensitive to small structural perturbations. In fact, despite much effort, only four native RFPs have been successfully monomerized, leaving the majority of RFP biodiversity untapped in biomarker development. Here we report the generation of monomeric variants o

SUBMITTER: Wannier TM 

PROVIDER: S-EPMC6275547 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature