Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells.

ABSTRACT: The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study i

SUBMITTER: Anisimov SV 

PROVIDER: S-EPMC6275915 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature