Metalloid Reductase of Pseudomonas moravenis Stanleyae Conveys Nanoparticle Mediated Metalloid Tolerance.

ABSTRACT: A glutathione reductase (GSHR)-like enzyme in Pseudomonas moraviensis stanleyae was previously implicated as underlying the bacterium's remarkable SeO₃ ^2- tolerance. Herein, this enzyme is sequenced, recombinantly expressed, and fully characterized. The enzyme is highly adapted for selenodiglutathione substrates (K _m = 336 μM) compared to oxidized glutathione (K _m = 8.22 mM). The recombinant expression of this enzyme in the laboratory strains of Escherichia coli conveys a 10-fold increase in IC₉₀ for SeO₃ ^2-. Moreover, selenium nanoparticles are observed when the enzyme is overexpressed in the cells exposed to SeO₃ ^2-, but not in the corresponding no-enzyme control

SUBMITTER: Nemeth R 

PROVIDER: S-EPMC6275949 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature