A Rapid and Low-Cost Pathogen Detection Platform by Using a Molecular Agglutination Assay.

ABSTRACT: Rapid and low-cost pathogen diagnostic approaches are critical for clinical decision-making procedures. Cultivating bacteria often takes days to identify pathogens and provide antimicrobial susceptibilities. The delay in diagnosis may result in compromised treatment and inappropriate antibiotic use. Over the past decades, molecular-based techniques have significantly shortened pathogen identification turnaround time with high accuracy. However, these assays often use complex fluorescent labeling and nucleic acid amplification processes, which limit their use in resource-limited settings. In this work, we demonstrate a wash-free molecular agglutination assay with a straightforward mixing and incubation step that significantly simplifies procedures of molecular testing. By targeting the 16S

SUBMITTER: Wu TF 

PROVIDER: S-EPMC6276042 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature