Proteomic analysis of insulin secretory granules in INS-1 cells by protein correlation profiling.

ABSTRACT:

Abstract

Insulin secretory granules (ISGs), a group of distinguishing organelles in pancreatic β cells, are responsible for the storage and secretion of insulin to maintain blood glucose homeostasis. The molecular mechanisms of ISG biogenesis, maturation, transportation, and exocytosis are still largely unknown because the proteins involved in these distinct steps have not been fully identified. Subcellular fractionation by density gradient centrifugation has been successfully employed to analyze the proteomes of numerous organelles. However, use of this method to elucidate the ISG proteome is limited by co-fractionated contaminants because ISGs are very dynamic and have abundant exchanges or contacts with other organelles, such as the Golgi apparatus, lysosomes, and endosomes. In

SUBMITTER: Li M 

PROVIDER: S-EPMC6276070 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature