Microglial TLR4-dependent autophagy induces ischemic white matter damage via STAT1/6 pathway.

ABSTRACT: Rationale: Ischemic white matter damage frequently results in myelin loss, accompanied with microglial activation. We previously found that directing microglia towards an anti-inflammatory phenotype provided a beneficial microenvironment and helped maintain white matter integrity during chronic cerebral hypoperfusion. However, the molecular mechanisms underlying microglial polarization remain elusive. Methods: Hypoperfusion induced white matter damage mice model and lipopolysaccharide (LPS) induced primary cultured microglia were established. Autophagy activation in microglia was detected both in vivo and in vitro by immunofluorescence, Western blot and electron microscopy. Autophagy inhibitors/agonist were administrated to investigate the role of autophagic pro

SUBMITTER: Qin C 

PROVIDER: S-EPMC6276098 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature