Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument.

ABSTRACT: Coupling of size-exclusion chromatography with biological solution small-angle X-ray scattering (SEC-SAXS) on dedicated synchrotron beamlines enables structural analysis of challenging samples such as labile proteins and low-affinity complexes. For this reason, the approach has gained increased popularity during the past decade. Transportation of perishable samples to synchrotrons might, however, compromise the experiments, and the limited availability of synchrotron beamtime renders iterative sample optimization tedious and lengthy. Here, the successful setup of laboratory-based SEC-SAXS is described in a proof-of-concept study. It is demonstrated that sufficient quality data can be obtained on a laboratory instrument with small sample consumption, comparable to typical synchrotron SEC-SA

SUBMITTER: Bucciarelli S 

PROVIDER: S-EPMC6276278 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature