High-throughput continuous rotation electron diffraction data acquisition via software automation.

ABSTRACT: Single-crystal electron diffraction (SCED) is emerging as an effective technique to determine and refine the structures of unknown nano-sized crystals. In this work, the implementation of the continuous rotation electron diffraction (cRED) method for high-throughput data collection is described. This is achieved through dedicated software that controls the transmission electron microscope and the camera. Crystal tracking can be performed by defocusing every nth diffraction pattern while the crystal rotates, which addresses the problem of the crystal moving out of view of the selected area aperture during rotation. This has greatly increased the number of successful experiments with larger rotation ranges and turned cRED data collection into a high-throughput method. The experimental

SUBMITTER: Cichocka MO 

PROVIDER: S-EPMC6276279 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature