High Throughput Screening of Additives Using Factorial Design to Promote Survival of Stored Cultured Epithelial Sheets.

ABSTRACT: There is a need to optimize storage conditions to preserve cell characteristics during transport of cultured cell sheets from specialized culture units to distant hospitals. In this study, we aimed to explore a method to identify additives that diminish the decrease in the viability of stored undifferentiated epidermal cells using multifactorial design and an automated screening procedure. The cultured cells were stored for 7-11 days at 12°C in media supplemented with various additives. Effects were evaluated by calcein staining of live cells as well as morphology. Twenty-six additives were tested using (1) a two-level factorial design in which 10 additives were added or omitted in 64 different combinations and (2) a mixture design with 5 additives at 5 different concentrations in a total

SUBMITTER: Reppe S 

PROVIDER: S-EPMC6276401 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature