Polyunsaturated fatty acid elongation and desaturation in activated human T-cells: ELOVL5 is the key elongase.

ABSTRACT: PUFAs are important constituents of membrane glycerophospholipids. However, changes in the capacities to incorporate and metabolize PUFAs when cells enter the cell cycle have not been thoroughly studied. In this study, differences in the incorporation and metabolism of exogenous PUFAs in resting and proliferating primary human T-cells and in the Jurkat cell line were measured. Overall, proliferating T-cells and Jurkat cells had a greater capacity to incorporate and elongate exogenous 18- and 20-carbon PUFAs compared with resting T-cells. Proliferating T-cells and Jurkat cells also showed a greater capacity to desaturate 18-carbon PUFA substrates. Consistent with these observations, a significant increase in the expression of fatty acid desaturase (FADS) 1, FADS2, and elongation of very lon

SUBMITTER: Robichaud PP 

PROVIDER: S-EPMC6277159 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature