Higher lipid turnover and oxidation in cultured human myotubes from athletic versus sedentary young male subjects.

ABSTRACT: In this study we compared fatty acid (FA) metabolism in myotubes established from athletic and sedentary young subjects. Six healthy sedentary (maximal oxygen uptake (VO_2max) ≤ 46 ml/kg/min) and six healthy athletic (VO_2max > 60 ml/kg/min) young men were included. Myoblasts were cultured and differentiated to myotubes from satellite cells isolated from biopsy of musculus vastus lateralis. FA metabolism was studied in myotubes using [¹⁴C]oleic acid. Lipid distribution was assessed by thin layer chromatography, and FA accumulation, lipolysis and re-esterification were measured by scintillation proximity assay. Gene and protein expressions were studied. Myotubes from athletic subjects showed lower FA accumulation, lower incorporation of FA into total lipids, t

SUBMITTER: Lund J 

PROVIDER: S-EPMC6277406 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature