Rapid characterization of secreted recombinant proteins by native mass spectrometry.

ABSTRACT: Characterization of overexpressed proteins is essential for assessing their quality, and providing input for iterative redesign and optimization. This process is typically carried out following purification procedures that require pronounced cost of time and labor. Therefore, quality assessment of recombinant proteins with no prior purification offers a major advantage. Here, we report a native mass spectrometry method that enables characterization of overproduced proteins directly from culture media. Properties such as solubility, molecular weight, folding, assembly state, overall structure, post-translational modifications and binding to relevant biomolecules are immediately revealed. We show the applicability of the method for in-depth characterization of secreted recombinant proteins f

SUBMITTER: Ben-Nissan G 

PROVIDER: S-EPMC6277423 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature