Genomic identification and functional analysis of essential genes in Caenorhabditis elegans.

ABSTRACT:

Background

Essential genes are required for an organism's viability and their functions can vary greatly, spreading across many pathways. Due to the importance of essential genes, large scale efforts have been undertaken to identify the complete set of essential genes and to understand their function. Studies of genome architecture and organization have found that genes are not randomly disturbed in the genome.

Results

Using combined genetic mapping, Illumina sequencing, and bioinformatics analyses, we successfully identified 44 essential genes with 130 lethal mutations in genomic regions of C. elegans of around 7.3 Mb from Chromosome I (left). Of the 44 essential genes, six of which were genes not characterized previously by mutant alleles, let-633/let-638 (B0261.1), let-12

SUBMITTER: Yu S 

PROVIDER: S-EPMC6278001 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature