RF/6A Chorioretinal Cells Do Not Display Key Endothelial Phenotypes.

ABSTRACT:

Purpose

The misuse of inauthentic cell lines is widely recognized as a major threat to the integrity of biomedical science. Whereas the majority of efforts to address this have focused on DNA profiling, we sought to anatomically, transcriptionally, and functionally authenticate the RF/6A chorioretinal cell line, which is widely used as an endothelial cell line to model retinal and choroidal angiogenesis.

Methods

Multiple vials of RF/6A cells obtained from different commercial distributors were studied to validate their genetic, transcriptomic, anatomic, and functional fidelity to bona fide endothelial cells.

Results

Transcriptomic profiles of RF/6A cells obtained either de novo or from a public data repository did not correspond to endothelial gene expression signatur

SUBMITTER: Makin RD 

PROVIDER: S-EPMC6278239 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature