Construction of a Quantitative Acetylomic Tissue Atlas in Rice (Oryza sativa L.).

ABSTRACT: PKA (protein lysine acetylation) is a key post-translational modification involved in the regulation of various biological processes in rice. So far, rice acetylome data is very limited due to the highly-dynamic pattern of protein expression and PKA modification. In this study, we performed a comprehensive quantitative acetylome profile on four typical rice tissues, i.e., the callus, root, leaf, and panicle, by using a mass spectrometry (MS)-based, label-free approach. The identification of 1536 acetylsites on 1454 acetylpeptides from 890 acetylproteins represented one of the largest acetylome datasets on rice. A total of 1445 peptides on 887 proteins were differentially acetylated, and are extensively involved in protein translation, chloroplast development, and photosynthesis, flowering

SUBMITTER: Li Z 

PROVIDER: S-EPMC6278296 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature