Identification of a 2'-O-Methyluridine Nucleoside Hydrolase Using the Metagenomic Libraries.

ABSTRACT: Ribose methylation is among the most ubiquitous modifications found in RNA. 2'-O-methyluridine is found in rRNA, snRNA, snoRNA and tRNA of Archaea, Bacteria, and Eukaryota. Moreover, 2'-O-methylribonucleosides are promising starting materials for the production of nucleic acid-based drugs. Despite the countless possibilities of practical use for the metabolic enzymes associated with methylated nucleosides, there are very few reports regarding the metabolic fate and enzymes involved in the metabolism of 2'-O-alkyl nucleosides. The presented work focuses on the cellular degradation of 2'-O-methyluridine. A novel enzyme was found using a screening strategy that employs Escherichia coli uracil auxotroph and the metagenomic libraries. A 2'

SUBMITTER: Aucynaite A 

PROVIDER: S-EPMC6278475 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature