Inhibition of protein disulfide isomerase induces differentiation of acute myeloid leukemia cells.

ABSTRACT: A cute myeloid leukemia is a malignant disease of immature myeloid cells. Despite significant therapeutic effects of differentiation-inducing agents in some acute myeloid leukemia subtypes, the disease remains incurable in a large fraction of patients. Here we show that SK053, a thioredoxin inhibitor, induces differentiation and cell death of acute myeloid leukemia cells. Considering that thioredoxin knock-down with short hairpin RNA failed to exert antiproliferative effects in one of the acute myeloid leukemia cell lines, we used a biotin affinity probe-labeling approach to identify potential molecular targets for the effects of SK053. Mass spectrometry of proteins precipitated from acute myeloid leukemia cells incubated with biotinylated SK053 used as a bait revealed protein disulfide is

SUBMITTER: Chlebowska-Tuz J 

PROVIDER: S-EPMC6278960 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature