Human antibody reaction against recombinant salivary proteins of Phlebotomus orientalis in Eastern Africa.

ABSTRACT:

Background

Phlebotomus orientalis is a vector of Leishmania donovani, the causative agent of life threatening visceral leishmaniasis spread in Eastern Africa. During blood-feeding, sand fly females salivate into the skin of the host. Sand fly saliva contains a large variety of proteins, some of which elicit specific antibody responses in the bitten hosts. To evaluate the exposure to sand fly bites in human populations from disease endemic areas, we tested the antibody reactions of volunteers' sera against recombinant P. orientalis salivary antigens.

Methodology/principal findings

Recombinant proteins derived from sequence data on P. orientalis secreted salivary proteins, were produced using either bacterial (five proteins) or mammalian (four proteins) expression systems and

SUBMITTER: Sumova P 

PROVIDER: S-EPMC6279015 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature