Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse.

ABSTRACT: Understanding factors that regulate zygotic genome activation (ZGA) is critical for determining how cells are reprogrammed to become totipotent or pluripotent. There is limited information regarding how this process occurs physiologically in early mammalian embryos. Here, we identify a mediator complex subunit, MED13, as translated during mouse oocyte maturation and transcribed early from the zygotic genome. Knockdown and conditional knockout approaches demonstrate that MED13 is essential for ZGA in the mouse, in part by regulating expression of the embryo-specific chromatin remodeling complex, esBAF. The role of MED13 in ZGA is mediated in part by interactions with E2F transcription factors. In addition to MED13, its paralog, MED13L, is required for successful preimplantation embryo devel

SUBMITTER: Miao YL 

PROVIDER: S-EPMC6279059 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature