Transcriptome profiling of individual rhesus macaque oocytes and preimplantation embryos.

ABSTRACT: Early mammalian embryonic transcriptomes are dynamic throughout the process of preimplantation development. Cataloging of primate transcriptomics during early development has been accomplished in humans, but global characterization of transcripts is lacking in the rhesus macaque: a key model for human reproductive processes. We report here the systematic classification of individual macaque transcriptomes using RNA-Seq technology from the germinal vesicle stage oocyte through the blastocyst stage embryo. Major differences in gene expression were found between sequential stages, with the 4- to 8-cell stages showing the highest level of differential gene expression. Analysis of putative transcription factor binding sites also revealed a striking increase in key regulatory factors in 8-cell e

SUBMITTER: Chitwood JL 

PROVIDER: S-EPMC6279092 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature