Loss of MYO5B Leads to Reductions in Na⁺ Absorption With Maintenance of CFTR-Dependent Cl^- Secretion in Enterocytes.

ABSTRACT:

Background & aims

Inactivating mutations in MYO5B cause microvillus inclusion disease (MVID), but the physiological cause of the diarrhea associated with this disease is unclear. We investigated whether loss of MYO5B results in aberrant expression of apical enterocyte transporters.

Methods

We studied alterations in apical membrane transporters in MYO5B-knockout mice, as well as mice with tamoxifen-inducible, intestine-specific disruption of Myo5b (VilCre^ERT2;Myo5b^flox/flox mice) or those not given tamoxifen (controls). Intestinal tissues were collected from mice and analyzed by immunostaining, immunoelectron microscopy, or cultured enteroids were derived. Functions of brush border transporters in intestinal mucosa were measured in Ussing chambers. We ob

SUBMITTER: Engevik AC 

PROVIDER: S-EPMC6279525 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature